1 Scope
This document specifies a method for the quantification of four tobacco specific nitrosamines ( TSNAs ) in the total particulate matter of cigarette mainstream smoke with the intense smoking
regime specified in ISO 20778 by using reversed phase high performance liquid chromatography with tandem mass spectrometry
(LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB)
and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).