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Typically, only two lanes of a gel are needed to demonstrate the specificity of a phosphoprotein-specific antibody: one for the de-phosphorylated sample and one for the control, an untreated sample....
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De-phosphorylation of DNAThe preferred method of de-phosphorylation uses the buffer system described by Pharmacia for most of their restriction endonucleases.You will need:10 x OPA+ Buffer (100mM Tris.acetate...
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