DIN EN 12821-2009
食品.高效液相色谱法测定维生素D.维生素D3和维生素D2的测量.德文版本EN 12821-2009

Foodstuffs - Determination of vitamin D by high performance liquid chromatography - Measurement of cholecalciferol (D₃) or ergocalciferol (D₂); German version EN 12821:2009

DIN EN 12821-2009 发布历史

This European Standard specifies a method for the determination of vitamin 03 (cholecalciferol) or vitamin D2 (ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC). Vitamin D3 is primary in foodstuffs of animal origin, while vitamin D2 is primary in wild mushrooms. Both vitamin D3 and vitamin D2 can be present in fortified foodstuffs. This European Standard is not applicable for samples with a content of vitamin D3 and vitamin D2. Apart from the vitamin D activity from the parent forms, vitamin D3 and vitamin D2, the corresponding metabolites 25-hydroxy vitamin D and 1,25-dihydroxy vitamin D also contribute to the vitamin D activity. This European Standard does only include measurement of vitamin D3 or vitamin D2. This European Standard provides the base for the analytical methods. It is intended to serve as a frame in which the analyst can define his own analytical work in accordance to the standard procedure. This method has been validated in inter-laboratory tests on fortified and non-fortified samples such as margarine, milk, milk powder, liquid infant formula, infant formula, cooking oil, and fish oil at levels from 0,4 μg/100 g to 14 μg/100 g. Further information on the validation data is given in Annex D.

DIN EN 12821-2009由德国标准化学会 DE-DIN 发布于 2009-08。

DIN EN 12821-2009 在中国标准分类中归属于: X04 基础标准与通用方法，在国际标准分类中归属于: 67.050 食品试验和分析的一般方法。

DIN EN 12821-2009 发布之时，引用了标准

• EN ISO 3696 分析实验室用水.规范和试验方法(ISO 3696-1987)

DIN EN 12821-2009的历代版本如下：

• 2009年08月 DIN EN 12821-2009 食品.高效液相色谱法测定维生素D.维生素D3和维生素D2的测量.德文版本EN 12821-2009

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标准号

DIN EN 12821-2009

发布日期

2009年08月

实施日期

废止日期

无

中国标准分类号

X04

国际标准分类号

67.050

发布单位

DE-DIN

引用标准

EN ISO 3696

被代替标准

DIN EN 12821-2000 DIN EN 12821-2007

适用范围

This European Standard specifies a method for the determination of vitamin 03 (cholecalciferol) or vitamin D2 (ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC). Vitamin D3 is primary in foodstuffs of animal origin, while vitamin D2 is primary in wild mushrooms. Both vitamin D3 and vitamin D2 can be present in fortified foodstuffs. This European Standard is not applicable for samples with a content of vitamin D3 and vitamin D2. Apart from the vitamin D activity from the parent forms, vitamin D3 and vitamin D2, the corresponding metabolites 25-hydroxy vitamin D and 1,25-dihydroxy vitamin D also contribute to the vitamin D activity. This European Standard does only include measurement of vitamin D3 or vitamin D2. This European Standard provides the base for the analytical methods. It is intended to serve as a frame in which the analyst can define his own analytical work in accordance to the standard procedure. This method has been validated in inter-laboratory tests on fortified and non-fortified samples such as margarine, milk, milk powder, liquid infant formula, infant formula, cooking oil, and fish oil at levels from 0,4 μg/100 g to 14 μg/100 g. Further information on the validation data is given in Annex D.

DIN EN 12821-2009 中可能用到的仪器设备