DIN ISO 16000-17-2010
室内空气.第17部分:霉菌的测定与计算.培养基方法(ISO 16000-17-2008)

Indoor air - Part 17: Detection and enumeration of moulds - Culture-based method (ISO 16000-17:2008)

DIN ISO 16000-17-2010 发布历史

Mould spores are widely distributed in the outdoor environment and, therefore, occur in varying concentrations also indoors. Growth of moulds in indoor environments, however, should be considered a hygienic problem because epidemiological studies have revealed that dampness and/or mould growth in homes and health problems affecting the occupants are closely related. Harmonized methods for sampling, detection and enumeration of moulds including guidelines for sampling strategies are important for comparative assessment of mould problems indoors. Sampling is carried out according to the method described in DIN ISO 16000-16 or -18. Agar plates (DG 18 agar and malt-extract agar or potato extrose agar) obtained from sampling by impaction are incubated directly at (25 +/- 3) ?C and (36 +/- 2) ?C. Filters obtained from sampling by filtration are re-suspended in saline solution (0, 9 % NaCl) with 0, 01 % Polysorbate 80. Decimal dilutions of the suspension are prepared and aliquots spread on DG 18 agar as well as on malt-extract agar or potato dextrose agar (indirect method). Agar plates are incubated at (25 +/- 3) ?C. For special purposes plates can be incubated at (36 +/- 2) ?C (e. g. thermotolerant Aspergillus spp.) or (45 +/- 2) ?C (Aspergillus fumigatus). After incubation, mould colonies are identified and counted.

DIN ISO 16000-17-2010由德国标准化学会 DE-DIN 发布于 2010-06-01。

DIN ISO 16000-17-2010 在中国标准分类中归属于: C51 环境卫生，在国际标准分类中归属于: 13.040.20 环境空气。

DIN ISO 16000-17-2010 发布之时，引用了标准

• ISO 8199 *， 2018-09-30 更新

* 在 DIN ISO 16000-17-2010 发布之后有更新，请注意新发布标准的变化。

DIN ISO 16000-17-2010的历代版本如下：

• 2010年06月01日 DIN ISO 16000-17-2010 室内空气.第17部分:霉菌的测定与计算.培养基方法(ISO 16000-17-2008)

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标准号

DIN ISO 16000-17-2010

发布日期

2010年06月01日

实施日期

废止日期

无

中国标准分类号

C51

国际标准分类号

13.040.20

发布单位

DE-DIN

引用标准

ISO 8199 ISO 16000-16 ISO 16000-18

被代替标准

DIN ISO 16000-17-2007 DIN ISO 16000-17-2010-01

适用范围

Mould spores are widely distributed in the outdoor environment and, therefore, occur in varying concentrations also indoors. Growth of moulds in indoor environments, however, should be considered a hygienic problem because epidemiological studies have revealed that dampness and/or mould growth in homes and health problems affecting the occupants are closely related. Harmonized methods for sampling, detection and enumeration of moulds including guidelines for sampling strategies are important for comparative assessment of mould problems indoors. Sampling is carried out according to the method described in DIN ISO 16000-16 or -18. Agar plates (DG 18 agar and malt-extract agar or potato extrose agar) obtained from sampling by impaction are incubated directly at (25 +/- 3) ?C and (36 +/- 2) ?C. Filters obtained from sampling by filtration are re-suspended in saline solution (0, 9 % NaCl) with 0, 01 % Polysorbate 80. Decimal dilutions of the suspension are prepared and aliquots spread on DG 18 agar as well as on malt-extract agar or potato dextrose agar (indirect method). Agar plates are incubated at (25 +/- 3) ?C. For special purposes plates can be incubated at (36 +/- 2) ?C (e. g. thermotolerant Aspergillus spp.) or (45 +/- 2) ?C (Aspergillus fumigatus). After incubation, mould colonies are identified and counted.

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