当温度上升至Diethyl Ether沸点时,Diethyl Ether开始沸腾,并形成蒸汽由连接管上升至冷凝器,蒸气遇冷而成液体,滴入提取管内将样品浸泡,样品的脂肪将逐渐溶于其中,当液面超过虹吸管上端时,溶有脂肪的Diethyl Ether经虹吸管流入提取瓶。调节水浴温度至50~60℃,使Diethyl Ether每小时循环3~5次,提取时间视样品性质而定,通常要12~16h。...
recrystallized bOG through a scintered glass funnel.7) Wash crystals with 500 ml ice cold diethyl ether.8) Completely dry crystals and scrape out of funnel.9) Weigh crystals and store as powder in the...
This interference can be eliminated by acetone precipitation of the protein, followed by delipidation with diethyl ether.MOLECULAR PROBES (C-6667)CBQCA Protein Quantitation Kit (pdf)10 ng/mL - 150 µg/...
Residual phenol then is removed by extraction with an equal volume of water-saturated diethyl ether....
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