In general, use LMP agarose if you wish to use the DNA for further manipulation.Freeze squeeze - Excise band and place pieces of it in a home-made spin-column (a piece of filter placed in a PCR 0.5ml eppendorf...
It is also acceptable to place the gel apparatus on two stirring blocks to recirculate the buffer....
To anneal, heat to 90OC and incubate for ~10 minutes. Cool slowly to RT. ...
and tested a 75 mm diameter grid-cooled sapphire window under 1 kw heat load.Fabricated and tested a grid-cooled sapphire window double window fixture in a realistic microwave apparatus.Assembled and...
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