ABCG1 antibody
Cat.#: 1945-1
Rabbit Monoclonal Antibody
Clone ID: EP1366Y
Swiss Prot: P45844
Mol Weight: 110kDa
Size: 100ul
Description
ATP-binding cassette (ABC) transporter ABCG1 (ABC8) is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Conversely, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1. Immunocytochemical and flow cytometric analyses revealed that ABCG1 is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. Results demonstrate a regulator function for ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages (1). ABC transporters belonging to the ABCG (White) subfamily (ABCG1, ABCG5, and ABCG8) have been shown to be critically involved in the regulation of lipid-trafficking mechanisms in macrophages, hepatocytes, and intestinal mucosa cells (2) Studies show that treatment with Rhodobacter sphaeroiders LPS, an LPS antagonist, or PD169316, a specific p38 MAP kinase inhibitor, prevented the induction of ABCA1 by LPS. Therefore, this suggests that both transport of LPS from the plasma membrane to an intracellular site and activation of p38 MAP kinase are involved in the LPS-mediated induction of ABCA1 (3).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:50 - 100
ICC: 1:100 - 250

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
 

Products Data

 

A. Western blot analysis on HUVEC cell lysate using anti-ABCG1 RabMAb (cat. # 1945-1), dilution 1:2000
B. Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-ABCG1 RabMAb (cat. #1945-1).





DescriptionATP-binding cassette (ABC) transporter ABCG1 (ABC8) is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Conversely, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1. Immunocytochemical and flow cytometric analyses revealed that ABCG1 is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. Results demonstrate a regulator function for ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages (1). ABC transporters belonging to the ABCG (White) subfamily (ABCG1, ABCG5, and ABCG8) have been shown to be critically involved in the regulation of lipid-trafficking mechanisms in macrophages, hepatocytes, and intestinal mucosa cells (2) Studies show that treatment with Rhodobacter sphaeroiders LPS, an LPS antagonist, or PD169316, a specific p38 MAP kinase inhibitor, prevented the induction of ABCA1 by LPS. Therefore, this suggests that both transport of LPS from the plasma membrane to an intracellular site and activation of p38 MAP kinase are involved in the LPS-mediated induction of ABCA1 (3).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:50 - 100
ICC: 1:100 - 250

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

 

 

Specificity
A synthetic peptide corresponding to residues on human ABCG1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ABCG1 antibody, ABC8 antibody, MGC34313 antibody, WHITE1 antibody, ATP-binding cassette sub-family G member 1 antibody, ATP-binding cassette transporter 8 antibody, White protein homolog antibody

Description References
1. Klucken J, et al. Proc Natl Acad Sci 97(2):817-22, 2000
2. Kaplan R, et al. J Lipid Res 43(6):952-9, 2002.
3. Schmitz G, et al. J Lipid Res 42(10):1513-20, 2001

Product References
1. Bogan RL, Hennebold JD, The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum ,Reproduction 139 (2010) 163-176
[Application: WB]
2. Field FJ, Watt K, Mathur SN, TNF-α decreases ABCA1 expression and attenuates HDL cholesterol efflux in the human intestinal cell line Caco-2 ,J Lipid Res 51 (2010) 1407-1415