Atg3(ATG3)antibody
Cat.#: 3580-1
Rabbit Monoclonal Antibody
Clone ID: EPR4801
Swiss Prot: Q9NT62
Mol Weight: 40kDa
Size: 100ul

Description
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole (1). Atg3 is an E2-like enzyme involved in autophagy and mitochondrial homeostasis. It catalyzes the conjugation of Atg8-like proteins to phosphatidylethanolamine (PE), which is essential to autophagy. Atg3 also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of Atg12 to itself; Atg12 conjugation to Atg3 plays a role in mitochondrial homeostasis but not in autophagy. Atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with Atg3 (2).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

Western blot analysis on (A) K562, (B) HL-60, (C) HeLa, and (D) Jurkat cell lysates using anti-Atg3 RabMAb (cat. #3580-1).
Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-Atg3 RabMAb (cat. #3580-1).
Immunofluorescent staining of HeLa cells using anti-Atg3 RabMAb (cat. #3580-1).

Specificity
A synthetic peptide corresponding to residues near the N-terminus in human Atg3 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATG3 antibody, APG3 antibody, APG3-LIKE antibody, APG3L antibody, DKFZp564M1178 antibody, FLJ22125 antibody, MGC15201 antibody, PC3-96 antibody, Ubiquitin-like-conjugating enzyme ATG3 antibody, Protein PC3-96 antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
2. The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010).