ATG4A antibody
Cat.#: 3194-1
Rabbit Monoclonal Antibody
Clone ID: EPR4122
Swiss Prot: Q8WYN0
Mol Weight: 45kDa
Size: 100ul

Description
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging (1). ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of target proteins, such as MAP1LC3, GABARAPL2 and GABARAP. ATG4A belongs to the peptidase C54 family, and is widely with high expression level in skeletal muscle and brain (2).

Recommended Applications
WB, IHC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data  

 

Western blot analysis on (A) fetal liver, (B) HepG2, (C) Jurkat, and (D) fetal brain lysates using anti-ATG4A RabMAb (cat. #3194-1).
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ATG4A RabMAb (cat. #3194-1).
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-ATG4A RabMAb (cat. #3194-1).

Specificity
A synthetic peptide corresponding to residues in human ATG4A was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATG4A antibody, APG4A antibody, AUTL2 antibody, Cysteine protease ATG4A antibody, AUT-like 2 cysteine endopeptidase antibody, Autophagin-2 antibody, Autophagy-related cysteine endopeptidase 2 antibody, Autophagy-related protein 4 homolog A antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1; 33: D54-8.
2. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2010)