Atg5(ATG5)antibody
Cat.#: 3167-1
Rabbit Monoclonal Antibody
Clone ID: EPR1755(2)
Swiss Prot: Q9H1Y0
Mol Weight: 32/55kDa
Size: 100ul

Description
Atg5 (Autophagy protein 5) is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Atg5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity (1).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

 

Western blot analysis on (A) Raji, (B) HeLa, (C) HT-1080, (D) fetal kidney, (E) C6, (F) Raw264.7, (G) PC-12, (H) NIH/3T3 cell lysates using anti-Atg5 RabMAb (cat. #3167-1).
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using anti-Atg5 RabMAb (cat. #3167-1).
Immunohistochemical analysis of paraffin-embedded human ovarian adenocarcinoma tissue using anti-Atg5 RabMAb (cat. #3167-1).

Specificity
A synthetic peptide corresponding to residues in human Atg5 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ATG5 antibody, APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, Autophagy protein 5 antibody, APG5-like antibody, Apoptosis-specific protein antibody

Description References
1. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2010)