Aurora-A(AURKA)antibody
Cat.#: 3858-1
Rabbit Monoclonal Antibody
Clone ID: EPR5026
Swiss Prot: O14965
Mol Weight: 46kDa
Size: 100ul

Description
Aurora-A is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Aurora-A is ubiquitously expressed and activated through an interaction with Ajuba and TPX2 during late G2 and mitotic phases. Activated form regulates the functions of centrosomes, spindles, and kinetochores required for normal mitotic progression. Initially localized at centrosome during interphase, Aurora-A is translocated to mitotic spindles in early mitotic phase and finally degraded after metaphase/anaphase transition (2). Overexpression of Aurora-A is seen various tumors; overexpression can induce abnormalities in G2 and spindle checkpoints, and cytokinesis failure (3).

Recommended Applications
WB, ICC, FC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100 - 250
FC: 1:100 - 500

Species Reactivity
Human, Mouse

Species cross-reactivity is based on WB analysis

Products Data  

 

Western blot analysis on (A) BXPC-3, (B) LnCaP, (C) SKBR-3, and (D) HepG2 cell lysates using anti-Aurora-A RabMAb (cat. #3858-1).
Immunofluorescent staining of HeLa cells using anti-Aurora-A RabMAb (cat. #3858-1).
Western blot analysis on Neuro-2a cell lysates using anti-Aurora-A RabMAb (cat. #3858-1).

Specificity
A synthetic peptide corresponding to residues in human Aurora-A was used as an immunogen.

Storage Condition and Buffer
Store at -20° 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
AURKA antibody, AIK antibody, ARK1 antibody, AURA antibody, AURORA2 antibody, BTAK antibody, MGC34538 antibody, STK15 antibody, STK6 antibody, STK7 antibody, Serine/threonine-protein kinase 6 antibody, Breast tumor-amplified kinase antibody, Serine/threonine-protein kinase 15 antibody, Serine/threonine-protein kinase aurora-A antibody

Description References
1. Katayama H, et al. Cancer Metastasis Rev. 22(4): 451-64, 2003
2. Duct D et al, Exp Cell Res. 15;301(1):60-7, 2004.
3. Murimoto T et al, Nat Rev Cancer. Jan;5(1):42-50, 2005.