Aldolase(ALDOA)Antibody(N-term)
Cat.#: T0891
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P04075
Mol Weight: 39kDa
Size: 100ug

Description
Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.

Recommended Applications
WB, IHC, ELISA

Applications and Recommended Dilution Factors
WB: 1:50~100
IHC: 1:10~50
ELISA: 1:1,000

Species Reactivity
Human

Products Data 

 

Western blot analysis of anti-ALDOA Antibody (N-term) in CEM cell line lysates (35ug/lane). ALDOA(arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specificity
This ALDOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73~103 amino acids from the N-terminal region of human ALDOA.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names
ALDOA antibody, ALDA antibody, GSD12 antibody, MGC10942 antibody, MGC17716 antibody, MGC17767 antibody, Fructose-bisphosphate aldolase A antibody, Lung cancer antigen NY-LU-1 antibody, Muscle-type aldolase antibody

Description References
Gizak,A., Proteins 72 (1), 209-216 (2008)
Lu,J., Biochem. Biophys. Res. Commun. 369 (3), 948-952 (2008)
Valis,K., Mol. Cell. Biochem. 311 (1-2), 225-231 (2008)