Aldolase B antibody
Cat.#: S3224
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P05062
Mol Weight: 34.76kDa
Size: 100ul

Description
Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq]

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:500-1:3000

Species Reactivity
Human

Other species have not been tested

Products Data

Sample (30ug of whole cell lysate)
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
diluted at 1:1000

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 84 and 298 of Aldolase B

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
ALDOB antibody, ALDB antibody, ALDO2 antibody, Fructose-bisphosphate aldolase B antibody, Liver-type aldolase antibody