20S Proteasome alpha2 antibody
Cat.#: S1136
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P25787
Mol Weight: 25.9kDa
Size: 100ug

Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq]

Recommended Applications
WB, ICC

Applications and Recommended Dilution Factors
WB: 1:500-1:3000
ICC: 1:100-1:200

Species Reactivity
Human

Other species have not been tested

Products Data

Sample(30ug of whole cell lysate)
A: H1299
B: HeLa S3
C: Hep G2
D: MOLT4
E: Raji
12% SDS PAGE
diluted at 1:1000

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 226 of 20S Proteasome alpha2

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
PSMA2 antibody, HC3 antibody, MU antibody, PMSA2 antibody, PSC2 antibody, Proteasome subunit alpha type-2 antibody, Macropain subunit C3 antibody, Multicatalytic endopeptidase complex subunit C3 antibody, Proteasome component C3 antibody