Acetyl CoA Carboxylase 1(ACC1)(ACACA)antibody
Cat.#: 3495-1
Rabbit Monoclonal Antibody

Clone ID: EPR4971
Swiss Prot: Q13085
Mol Weight: 265kDa
Size: 100ul

Description
Acetyl-CoA Carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Recommended Applications
WB, IHC, ICC, FC

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:250 - 500
ICC: 1:100 - 250
FC: 1:100 - 500

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data

 

Western blot analysis on (A) 293T, (B) HepG2, and (C) SH-SY5Y cell lysates using anti-Acetyl CoA Carboxylase 1 (ACC1) RabMAb (cat. #3495-1).
Immunohistochemical analysis of paraffin-embedded brain tissue using anti-Acetyl CoA Carboxylase 1 (ACC1) RabMAb (cat. #3495-1).
Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using anti-Acetyl CoA Carboxylase 1 (ACC1) RabMAb (cat. #3495-1).

Immunofluorescent staining of 293 cells using anti-Acetyl CoA Carboxylase 1 (ACC1) RabMAb (cat. #3495-1).

Specificity
A synthetic peptide corresponding to residues in N-terminus of human Acetyl CoA Carboxylase 1 (ACC1) was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACACA antibody, ACAC antibody, ACC antibody, ACC1 antibody, ACCA antibody, Biotin carboxylase antibody, ACC-alpha antibody

Description References
1. Jianqiang M, et al. PNAS 100:7515-7520, 2003
2. Girad J, et al. The FASEB Journal 8:36-42, 1994
3. Wakil SJ, et al. Annu Rev Biochem 52:537-539
4. Abu-Elheiga L, et al. Science 291: 2613-2616. 2001