DNA连接与转化 快速细胞破碎法实验步骤 挑取转化平板上的单菌接种于含相应抗生素的2ml LB中,37℃振荡培养至A590值为0
ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 130 mmMedium: 165 x 200 mmLarge: 165 x 260 mm5% Analytical GelsReagent1 mm Small1 mm Medium1 mm Large10X TBE Buffer (ml)33
目的研究SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示小分子多肽的方法
You will need the following reagents: 5x Sample Buffer 10% w/v SDS 10 mM Dithiothreitol, or beta-mercapto-ethanol 20 % v/v Glycerol 0
SDS-PAGE (Section 2.2) is probably the most commonly used gel electrophoretic system for analyzing proteins.
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