不列颠哥伦比亚大学的Bernhardt等（2017），则利用200微升的高通量溶解氧测量技术，对浮游植物细胞光照条件下的放氧量及黑暗条件下的氧气消耗量进行了测量分析，并计算其质量归一化代谢率（氧通量/总细胞体积）和光合作用的活化能。实验中使用了透明的PCR膜密封呼吸室，在3小时内每隔15秒测量一次氧气浓度（参见下图）。
英国约克大学的Prendergast-Miller等（2017），借助高通量呼吸测量技术对土壤微生物耗氧率进行测量进而对土壤质量进行快速评估。该研究小组使用600微升的高通量呼吸测量系统，通过实时测量不同土壤类型、蚯蚓处理及C源供给的土壤悬浮液的子样品的2小时内的氧气消耗，发现蚯蚓能够改变土壤微生物结构，进而影响土壤微生物的耗氧率。
参考文献：
Szela
T L, Marsh A G. Microtiter plate, optode respirometry, and
inter-individual variance in metabolic rates among nauplii of Artemia
sp[J]. Marine Ecology Progress, 2005, 296:281-289.
Köster M, Krause
C, Paffenhöfer G. Time-series measurements of oxygen consumption of
copepod nauplii[J]. Marine Ecology Progress, 2008, 353(01):157-164.
Flynn
E E, Todgham A E. Thermal windows and metabolic performance curves in a
developing Antarctic fish.[J]. Journal of Comparative Physiology B,
2017, 188(2):1-12.
Serrano X M, Miller M W, Hendee J C, et al.
Effects of thermal stress and nitrate enrichment on the larval
performance of two Caribbean reef corals[J]. Coral Reefs, 2018,
37(1):173-182.
Bernhardt J, Sunday J M, O'Connor M I. An empirical
test of the temperature dependence of carrying capacity[J]. bioRxiv,
2017: 210690.
Prendergastmiller M T, Thurston J, Taylor J, et al.
Rapid determination of soil quality and earthworm impacts on soil
microbial communities using fluorescence-based respirometry[C]// EGU
General Assembly Conference. EGU General Assembly Conference Abstracts,
2017.
Mclamore E S, Garland J L, Mackowiak C, et al. Development and
validation of an open source O2-sensitive gel for physiological
profiling of soil microbial communities[J]. J Microbiol Methods, 2014,
96(1):62-67.
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