Ratio Distortion with Isobaric Multiplexing

Problem: Quantitation of low-abundance proteins in a complex background is distorted by co-isolated interfering precursor ions

Synchronous Precursor Selection (SPS) for Accurate Quantification

TMT³ Experiment, Powered by SPS

Co-isolation of Interfering Ions Affects Accuracy

Results: Best possible accuracy and precision by reducing co-isolated interfering ions.

Enhanced Differences Using SPS MS3 Quantitation

Thermo Poster Note : Towards Mechanism of EGFR Inhibitor Resistance in Non-Small Lung Cancer Cell;M.Blank. et al

…While Still Getting Proteome Coverage

The speed and parallelizable work flow of the Orbitrap Fusion means not choosing between accuracy and coverage…

The Orbitrap Fusion using multinotch can Quantify more proteins than were Identified on the previous generation top tier hybrid

…Don’t Settle

Orbitrap Fusion Lumos Tribrid Mass Spectrometer

2015

Unmatched Analytical Performance
Revolutionary performance
Exceptional versatility
Unprecedented usability
Highest sensitivity