二、细胞实验效果不错，那动物实验怎么做？
动物模型可在在肿瘤基础性研究和抗肿瘤研究中发挥重要作用。结直肠癌研究中动物实验主要分为三种类型：I自发结直肠癌模型；II诱导结直肠癌模型；III 移植结直肠癌模型。第I种主要是通过构建转基因动物的方式实现，第II种则利用化学诱导剂诱导结直肠癌发生，但是诱导模型不便于研究肿瘤的侵袭和转移能力；第III种将人类肿瘤组织或细胞直接接种于裸鼠进行研究。因裸鼠培育条件低，无毛，易于动态观察肿瘤的生长状态，且通过接种方式、部位的选择可达到较满意的转移效果，因此目前多选择裸鼠进行人结直肠癌细胞异体移植。
据数据统计，约30%的CRC患者发生肝脏转移，这也是造成CRC患者死亡的最主要原因。今天我们就来着重看一看，结直肠癌转移模型的构建吧。
注：增殖方向体内模型为裸鼠皮下成瘤，属于肿瘤研究的常用模型，今天就不做详细介绍了。实验方法可参考前两期肺癌、胃癌的总结文章。
三、体内外模型选好了，那么我的实验需要使用哪种慢病载体对基因进行操作呢？
启动子、荧光标记、抗性标签是我们选择载体需要考虑的三大要素。实验不同，这些元件也需要相应变化。对于肠癌研究来说，人肠上皮细胞表面抗原基因A33启动子，可作为组织特异性启动子使用。按例祭出神图一张，定制你的专属载体，就是这么简单！
参考文献
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【10】 Huang, Huang, et al. “Regulation of TWIK-related potassium channel-1 (Trek1) restitutes intestinal epithelial barrier function.” Cellular & molecular immunology 13.1 (2016): 110-118.
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