Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation
Abstract: A rapid and efficient microwave-assisted solid-phase synthesis method is described for the preparation of thenonapeptideWDTVRISFK, using conventional Fmoc/But orthogonal
protection strategy. The synthesis protocol is based on theuse of
cycles of pulsed microwave irradiation with intermittent cooling of the
reaction during the removal of the Fmoc protectinggroup and during the
coupling. The desired nonapeptide was obtained in highest yield and
purity by employing MicroKantechnology.
The chemical reactions were carried out in a single-mode microwave
reactor, equipped with a fiber-optic probe tomonitor the reaction
temperature continuously.
摘要：本文描述一种快速有效的微波辅助固相合成方法，用以合成序列为WDTVRISFK的短肽，使用传统的Fmoc（9-芴氧羰基）/tBu（叔丁基）保护基策略。该合成方法是基于反应中的周期性脉冲微波辐射和间歇性冷却技术，在Fmoc保护基的脱除及偶联反应中均应用此技术。在应用微反应器技术后得到了高纯度和高收率的目标多肽。该反应在一个CEM单模微波反应器中进行，并且使用光纤进行连续测温。
原文出处：
Rapid solid-phase peptide synthesis using thermal andcontrolled microwave irradiation
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