Plant Cell是美国植物生物学家学会出版的生命科学领域学术刊物，在SCI植物类非评论性杂志中排名第一。
最近，Plant Cell发表了美国能源部植物研究实验室的文章，使用Wes定量western blot技术研究莱茵衣藻(Chlamydomonas reinhardtii)环境应力下类囊体膜重构。Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas，doi:10.1105/tpc.17.00446。
样本
莱茵衣藻样品使用2X Laemmli buffer提取
Wes定量western blot技术
在植物和藻类中，嵌入在极性脂基质中的光合复合物构成了光合作用所必需的类囊体膜的基本结构。为了检验这种可能性，我们进行了定量毛细管电泳和免疫检测(定量western blot技术）。
原文：To test this possibility, we performed quantitative capillary electrophoresis coupled with immuno-detection on the PL dw15-1, pgd1 mutant and complemented line G3.
定量western blot结果
1. 所有结果提供全膜结果
2. 小至8KD小分子量蛋白质检测无忧
3. 化学发光信号值Chemiluminescences直接定量
方法学原文
Immunoblotting
Following manufacturer''s instructions, Wes (simple Western system, ProteinSimple) and the 12-230 kDa Master Kit (ProteinSimple) were used to measure protein abundance with Agrisera antibodies. These included PsbA (D1 protein of PSII, C-terminal, catalog number AS01 016, dilution 1:300), PsbO (oxygen evolving complex of PSII, catalog number AS06 142-33, dilution 1:150), Lhcb2 (LHCII type II chlorophyll a/b binding protein, catalog number AS01 003, dilution 1:300), Lhcb6 (LHCII chlorophyll a/b binding protein CP24, catalog number AS01 010, dilution 1:200), PsaC (PSI-C, subunit of PSI, catalog number AS10 939, dilution 1:650),PsaD (PSI-D, subunit of PSI, catalog number AS09 461, dilution 1:150), Cyt f (cytochrome f subunit of cytochrome b6f complex, catalog number AS06 119, dilution1:6,500), Rieske (Rieske iron-sulfur protein of cytochrome b6f complex, catalog number AS08 330, dilution 1:650), and ATPase (ATP synthase, whole enzyme, catalog number AS08 370, dilution 1:500). Chemiluminescences of the samples were converted into gel like images with Compass software (ProteinSimple) or electropherograms using Origin for high-quality graphs.
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