今年7月,陈老湿统计过上半年Incucyte® 国内文章,并进行了详细的总结(点击查看)。第三季度,国内Incucyte® 相关文章数量依然延续了上半年的强劲势头,不完全统计数量就近40篇。
Incucyte® 实时活细胞分析系统不仅仅是一台分析仪器,更是一种活细胞成像的概念!它可以实时、高效的获取活细胞信息。从众多已发表的文章来看,Incucyte® 在细胞生物学中拥有广泛的应用,如:细胞增殖和细胞活性,划痕(细胞迁徙),杀伤实验,3D细胞实验等。
Q3国内 Incucyte® 文献一览表
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Novel hKDR mouse model depicts the antiangiogenesis and apoptosis-promoting effects of neutralizing antibodies targeting vascular endothelial growth factor receptor 2
中南大学
细胞黏附实验
Cinnamic aldehyde induces apoptosis of breast cancer cells via STAT3/cMyc pathway
DOI: https://doi.org/10.21203/rs.3.rs-2058567/v1
Chengdu University of Traditional Chinese Medicine
划痕
Regional gain and global loss of 5-hydroxymethylcytosine coexist in genitourinary cancers and regulate different oncogenic pathways
Clinical Epigenetics volume 14, Article number: 117 (2022)
Beijing Institute of Genomics
细胞增殖
A Novel tiRNA-Gly-GCC-1 Promotes Progression of Urothelial Bladder Carcinoma and Directly Targets TLR4
Cancers 2022, 14(19), 4555
Capital Medical University
细胞增殖
Caspase-8 mutants activate Nrf2 via phosphorylating SQSTM1 to protect against oxidative stress in esophageal squamous cell carcinoma
Free Radical Biology and Medicine Volume 192, 1 November 2022, Pages 51-62
Chinese Academy of Medical Sciences and Peking Union Medical College
细胞增殖
Oncogenic β-catenin stimulation of AKT2–CAD-mediated pyrimidine synthesis is targetable vulnerability in liver cancer
PNAS
Chinese Academy of Medical Sciences and Peking Union Medical College
细胞增殖
Combined Chemo–Immuno–Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform
ACS Appl. Mater. Interfaces 2022,
北京中医药大学
细胞增殖
Optogenetic control of GGGGCC repeat-containing RNA phase transition
Fundamental Research
复旦大学
细胞凋亡
LncRNA miR663AHG represses the development of colon cancer in a miR663a expression-dependent negative feedback loop
Peking University Cancer Hospital and Institute
细胞增殖
MAP7D2 reduces CD8+ cytotoxic T lymphocyte infiltration through MYH9-HMGB1 axis in colorectal cancer
Molecular Therapy
The Sixth Affiliated Hospital, Sun Yat-sen University
细胞增殖
A Positron Emission Tomography Tracer Targeting the S2 Subunit of SARS-CoV-2 in Extrapulmonary Infections
Mol. Pharmaceutics 2022
The Fifth Affiliated Hospital, Sun Yat-sen University
细胞活性
Multi-omics analysis of an in vitro photoaging model and protective effect of umbilical cord mesenchymal stem cell-conditioned medium
Stem Cell Research & Therapy volume 13
军科院
划痕
Computational prediction and CRISPR-Cas12a knockout and rescue assays identify many functionally conserved lncRNAs from zebrafish to human
DOI: https://doi.org/10.21203/rs.3.rs-1920866/v1
清华大学
细胞增殖
ACOT7 protects epidermal stem cells against lipid peroxidation
In Vitro Cellular & Developmental Biology - Animal volume 58
山东大学
细胞增殖
Modulation of intracellular kinase signaling to improve TIL stemness and function for adoptive cell therapy
https://doi.org/10.1002/cam4.5095
复旦大学
细胞杀伤
The Janus-faced role of TRPM2-S in retroperitoneal liposarcoma via increasing ROS levels。
Cell Communication and Signaling volume 20
Peking University Eighth School of Clinical Medicine
细胞增殖
EGFR blockade confers sensitivity to cabozantinib in hepatocellular carcinoma
Cell Discovery volume 8, Article number: 82 (2022)
细胞增殖
Rigid, eco-friendly and superhydrophobic SiO2-Polyvinyl alcohol composite sponge for durable oil remediation
Chemosphere
Volume 307, Part 4, November 2022
Third Institute of Oceanography Ministry of Natural Resources
细胞增殖
Protocatechuic acid and quercetin attenuate ETECcaused IPEC-1 cell inflammation and injury associated with inhibition of necroptosis and pyroptosis signaling pathways
DOI: https://doi.org/10.21203/rs.3.rs-1913405/v1
湖北工业大学
细胞增殖
Discovery of a Highly Potent and Selective Dual PROTAC Degrader of CDK12 and CDK13
J. Med. Chem. 2022, 65, 16
暨南大学
细胞增殖
ADAMTS10 inhibits aggressiveness via JAK/STAT/c-MYC pathway and reprograms macrophage to create an anti-malignant microenvironment in gastric cancer
Gastric Cancer (2022)
The Sixth Affiliated Hospital, Sun Yat-sen University
细胞增殖
Helicobacter pylori infection upregulates miR-135b5p to stimulate gastric cancer cell proliferation and inhibit cell apoptosis through activated IL-6/STAT3 signaling
research gate
Beijing Friendship Hospital
细胞增殖
Docetaxel-loaded M1 macrophage-derived exosomes for a safe and efficient chemoimmunotherapy of breast cancer
Journal of Nanobiotechnology volume 20
南通大学
细胞增殖
The RNA-binding protein PCBP1 represses lung adenocarcinoma progression by stabilizing DKK1 mRNA and subsequently downregulating β-catenin
Journal of Translational Medicine volume 20,
Chinese Academy of Medical Sciences and Peking Union Medical College
划痕
New Monoterpene-Conjugated Phenolic Constituents from Nutmeg and Their Autophagy Modulating Activities
J. Agric. Food Chem. 2022, 70
澳门科技大学
细胞增殖
Effect of semaphorin 3A on myoblast function and its transcriptional regulation mechanism
DOI: 10.11855/j.issn.0577-7402.2022.07.0701
解放军医学院
细胞增殖
KDM2A plays a dual role in regulating the expression of malignancy-related genes in esophageal squamous cell carcinoma
Biochemical and Biophysical Research Communications
Volume 624, 8 October 2022, Pages 53-58
西南医科大学
划痕
Dual Inhibition of CDK12/CDK13 Targets Both Tumor and Immune Cells in Ovarian Cancer
Cancer Res (2022) 82 (19): 3588–3602.
Ren Ji Hospital
细胞增殖
DNA methylation subtypes guiding prognostic assessment and linking to responses the DNA methyltransferase inhibitor SGI-110 in urothelial carcinoma
BMC Medicine volume 20
Peking University First Hospital
划痕
High-fat diet alleviates colitis by inhibiting ferroptosis via solute carrier family seven member 11
The Journal of Nutritional Biochemistry
Peking University Shenzhen Hospital
细胞凋亡
The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway
Int. J. Mol. Sci. 2022, 23(19),
the Sixth Affiliated Hospital, Sun Yat-sen University
划痕
TTC22 promotes m6A-mediated WTAP expression and colon cancer metastasis in an RPL4 bindingdependent pattern
DOI: https://doi.org/10.21203/rs.3.rs-1278091/v1
Peking University Cancer Hospital and Institute
划痕
Lycium barbarum L. and Salvia miltiorrhiza Bunge protect retinal pigment epithelial cells through endoplasmic reticulum stress
Journal of Ethnopharmacology Volume 296, 5 October 2022, 115519
The First Affiliated Hospital of Hunan University of Chinese Medicine
细胞增殖
Isobavachalcone disrupts mitochondrial respiration and induces cytotoxicity through ROS accumulation and Akt suppression Toxicon
Volume 216, September 2022, Pages 28-36
Tianjin University of Traditional Chinese Medicine
细胞增殖
Low concentration triphenyl phosphate fuels proliferation and migration of hepatocellular carcinoma cells
https://doi.org/10.1002/tox.23609
Zhujiang Hospital, Southern Medical University
细胞增殖
An optimized method for obtaining clinical-grade specific cell subpopulations from human umbilical cord-derived mesenchymal stem cells
https://doi.org/10.1111/cpr.13300
Beijing Institute of Radiation Medicine
细胞增殖
今天,陈老湿将选取几篇有意思的研究和大家一同分享:
暨南大学
Incucyte® + Octet® 双剑合璧
三阴性乳腺癌(Triple negative breast cancer, TNBC)是乳腺癌中最为棘手的一类。不断有研究表明,CDK12/13 是 TNBC 治疗的潜在治疗靶点。2022年8月发表在药物化学TOP期刊 Journal of Medicinal Chemistry 杂志上的一篇文章[1],通过采用蛋白水解靶向嵌合体 (PROTAC) 技术发现的第一系列高效和选择性双 CDK12/13 降解剂。其中最优化合物 7f 降解 CDK12 和 CDK13 的 DC50 值分别为 2.2nM 和 2.1 nM。化合物 7f 表现出对 CDK12/13 的高选择性,代表了进一步开发CDK12/13降解物作为TNBC患者的新靶向治疗的潜在先导分子。Octet® 验证7f来源化合物与CDK12 和 CDK13亲和力,Kd 值分别为 16.2 和 8.6 nM。Incucyte® 揭示化合物 7f可以显著抑制细胞增殖。本文用Incucyte® 和Octet® 分别在细胞水平和分子水平对小分子活性进行了验证。
降解剂 7f或DNA合成抑制剂(顺铂)均对MDA-MB-231细胞有中等程度的生长抑制作用。而7f与顺铂联合治疗可显著抑制细胞增殖。7f与PARP抑制剂奥拉帕尼(Olaparib)联合治疗也观察到类似的协同效应。
Octet® 检测7f来源化合物与CDK12和CDK13的亲和力,发现亲和力在nM。
复旦大学附属妇产科医院
TIL细胞杀伤:
Incucyte® 细胞杀伤实验好帮手
TIL(Tumor Infiltrating Lymphocytes,肿瘤浸润淋巴细胞)细胞疗法,主要依赖于肿瘤内部或附近的淋巴细胞已经具有识别和杀伤肿瘤细胞的能力,通过体外分离扩增并回输至病人体内,可以扩大免疫应答,加强肿瘤杀伤作用。但这种疗法的疗效可能会因 "容易耗尽 "而受到影响,体内持久性差而影响疗效。复旦大学附属妇产科医院发现抑制 PI3K-AKT 信号通路可以增强 TIL 的细胞毒性,可以改善当前基于 TIL 治疗实体瘤的前景的策略。Incucyte® 可以验证TIL杀伤能力。
2名宫颈癌患者的TIL细胞用 AKT和PIK3抑制剂处理,分别与Hele细胞和自体肿瘤细胞(ATC)共培养。与TIL对照组相比,具有AKT抑制作用的 TIL 对 HeLa 和 ATC 的细胞毒性显着增强。相比之下,PI3K抑制仅一位供体的TIL 对HeLa 细胞的细胞毒性有所改善,对 ATC 的杀伤没有显著影响。
自然资源部第三海洋研究所(海洋三所)
材料生物相容性:
Incucyte® 交叉科学好工具
石油被誉为“工业的血液”,石油在采集和运输的过程中都有泄露的风险,因此需要开发一些生态友好型吸附材料来处理石油溢漏。海洋三所开发了一种聚乙烯醇复合材料(H-SiO2-G-PVA)海绵,来解决这个问题。Incucyte® 用于评估该海绵的生物相容性,即将细胞长时间在该材料中培养并观察其生长情况。
在L929细胞中海绵组的细胞形态是纤维状的,与空白对照组一致。培养24小时和48小时后,海绵组的细胞存活率分别为95.20%和91.85%。对照组的相比,证实了海绵组具有出色的生物相容性。
为什么用Incucyte® 发表的文章越来越多呢?
培养箱内可长达数周的连续观察,最短几分钟间隔拍摄;大大减少人力操作,并且不像流式需要对细胞进行预处理,使得结果更准确。
6个板位,分别独立设置检测程序,可以兼容各种孔板和培养皿,通量高;Incucyte® 一次可以完成所有实验组实验。
高效简便的模块化软件设置和数据分析,输出图片、视频、生长曲线等多指标多参数。
大于100种优化过的活细胞专用荧光试剂、耗材及详尽的Protocol;文章数大于12000篇,引导长时间活细胞成像产品概念。
-参考文献-
Discovery of a Highly Potent and Selective Dual PROTAC Degrader of CDK12 and CDK13
J. Med. Chem. 2022, 65, 16
Modulation of intracellular kinase signaling to improve TIL stemness and function for adoptive cell therapy. Cancer Medicine. 2022;00:1–15.
Rigid, eco-friendly and superhydrophobic SiO2-Polyvinyl alcohol composite sponge for durable oil remediation. Chemosphere 307 (2022) 135990