服务流程: ★根据研究目的决定研究方案; ★DNA序列T克隆; ★DNA生物素探针合成(标记方法:Biotin-14-CTP) Biotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP (1). The biotinylated RNA can be used as a probe in membrane-based or in situ hybridizations (2). The amount of material provided is sufficient for up to 20 transcription reactions (1 µg template).; ★DNA-pull dwon实验; ★Western Blot检验(若需质谱分析请另外咨询; ★数据分析。