Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration by MPN technique with pre-enrichment
This part of ISO 21528 specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to. products intended for human consumption and the feeding of animals, and. environmental samples in the area of food production and food handling.Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C(or 30 °C)1) in liquid medium.This method is applicable. when the microorganisms sought are expected to need resuscitation before enrichment, and. when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.A limitation on the applicability of this