15％胶
水1.11.872.33.43.854.65.76.99.211.5
30％聚丙烯酰胺混合液2.54.2557.58.751012.5152025
1.5M Tris(pH=8.8)1.32.212.53.84.5556.37.51012.5
10% SDS0.050.0850.10.150.1750.20.250.30.40.5
10% AP0.050.0850.10.150.1750.20.250.30.40.5
TEMED0.0020.00340.0040.0060.0070.0080.010.0120.0160.02
积层胶配方
1ml2ml3ml4ml5ml6ml8ml10ml15ml
水0.681.42.12.73.44.15.56.810.2
30％聚丙烯酰胺混合液0.170.330.50.670.8311.31.72.55
1M Tris(pH=6.8)0.130.250.380.50.630.7511.251.95
10% SDS0.010.020.030.040.050.060.080.10.15
10% AP0.010.020.030.040.050.060.080.10.15
TEMED0.0010.0020.0030.0040.0050.0060.0080.010.015
PCR体系配方
Taq PCR
30ul system
123456789101112131415
10×buffer369121518212427303336394245
MgCl21.83.65.47.2910.812.614.416.21819.821.623.425.227
dNTP1.22.43.64.867.28.49.610.81213.214.415.616.818
primers1.22.43.64.867.28.49.610.81213.214.415.616.818
Template123456789101112131415
Taq0.30.60.91.21.51.82.12.42.733.33.63.94.24.5
ddH2O21.54364.586107.5129150.5172193.5215236.5258279.5301322.5
pfu PCR
50ul system
12345678910
10×pfu buffer5101520253035404550
dNTP2468101214161820
Template2468101214161820
primers12345678910
pfu0.511.522.533.544.55
ddH2O39.579119158198237277316356395
10×TBS
Tris 24.4g
NaCl 80g
定容至1L，调pH至7.6
1×TTBS: 10×TBS稀释后1L加1ml吐温
10×电转缓冲液
Tris 30.2g
Glycine 150g
定容至1L ，PH 8.5
Phosphate (Sodium) buffer Chart
Phosphate (Sodium) buffer Chart
Stock solution A
2 M monobasic sodium phosphate, monohydrate (276g/L) 一水磷酸二氢钠
Stock solution B
2 M dibasic sodium phosphate (284 g/L). 磷酸氢二钠
Mixing an appropriate volume (ml) of A and B as shown in the table below
and diluting to a total volume of 200 ml, a 1 M phosphate buffer of the
required pH at room temperature.
A B pH
92.0 8.0 0.8
90.0 10.0 5.9
87.7 12.3 6.0
85.5 15.0 6.1
81.5 19.5 6.2
77.5 22.5 6.3
73.5 26.5 6.4
68.5 31.5 6.5
62.5 37.5 6.6
56.5 43.5 6.7
51.0 49.0 6.8
45.0 55.0 6.9
39.0 61.0 7.0
33.0 67.0 7.1
28.0 72.0 7.2
23.0 77.0 7.3
19.0 81.0 7.4
16.0 84.0 7.5
13.0 87.0 7.6
10.5 89.5 7.7
8.5 91.5 7.8
Preparation of 0.1M potassium phosphate buffer at 25ºC
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