【解决方案】艾杰尔-飞诺美Kinetex色谱柱助力制药检测

LC/UV Conditions

Column: Kinetex 2.6 μm EVO C18

Dimensions: 100 x 4.6 mm

Part No.: 00D-4725-E0

Mobile Phase: 

A: 0.1 % H3PO4 in Water

B: 0.1 % H3PO4 in Acetonitrile

Gradient: 

Flow Rate: 1.75 mL/min

Temperature: Ambient

Detection: UV @ 254 nm

Sample: 1. Cefaclor

Sample Pretreatment

Reconstitute 1 mg of neat Cefaclor standard with 1 mL of 1 M

HCl. Heat at 60 ºC for 6 hours. Neutralize with 1 mL of 1 M NaOH.

Refrigerate until ready to inject.

Column:Kinetex 5 μm F5

Dimensions: 150 x 4.6 mm

Part No.: 00F-4724-E0

Mobile Phase: 

20 mM Potassium Phosphate pH 2.5/Acetonitrile (85:15)

Gradient: 

Injection Volume: 1 μL

Flow Rate: 1 mL/min

Temperature: 22 °C

Detection: UV @ 254 nm

Analytes: 

1. Cefadroxil

2. Cefaclor

3. Cefalexin

4. Cephradine

Column: 

Kinetex 2.6 μm PS C18

Dimensions: 50 x 2.1 mm

Mobile Phase: 

A: Water with 0.1 % Formic Acid

B: Acetonitrile with 0.1 % Formic Acid

Gradient: 

Flow Rate: 0.5 mL/min

Temperature: Ambient

Detector: MS/MS SCIEX™ API 4000™

Sample: 

22 Antibiotics (Retention on Kinetex PS C18)

1. Sulfaguanidine (0.48 min)

2. Lincomycin (1.86 min)

3. Sulfapyridine (2.16 min)

4. Sulfathiazole (2.19 min)

5. Marbofloxacin (2.19 min)

6. Sulfamerazine (2.25 min)

7. Ciprofloxacin (2.28 min)

8. Florfenicol (2.34 min)

9. Danofloxacin (2.34 min)

10. Enrofloxacin (2.36 min)

11. Sulfamonomethoxine (2.42 min)

12. Difloxacin (2.47 min)

13. Valnemulin (2.49)

14. Tylosin A (2.74 min)

15. Sulfamethoxazole (2.9 min)

16. Tiamulin (3.04 min)

17. Sulfaquinoxaline (3.16 min)

18. Trimethoprim (3.33 min)

19. Oxolinic Acid (3.42 min)

20. Flumequine (3.42 min)

21. Sulfadimethoxine (4.0 min)

22. Rifaximin (4.03 min)

Column: 

Kinetex^® 2.6 μm PS C18

Dimensions: 50 x 4.6 mm

Mobile Phase: 

A: Water with 20 mM Potassium

Dihydrogen Phosphate (pH 4.6)

B: Acetonitrile

Gradient: 

Flow Rate: 1.5 mL/min

Temperature: 23 °C

Detector: UV @ 235 nm

Sample: 1. Berberine