LC/UV Conditions
Column: Kinetex 2.6 μm EVO C18
Dimensions: 100 x 4.6 mm
Part No.: 00D-4725-E0
Mobile Phase:
A: 0.1 % H3PO4 in Water
B: 0.1 % H3PO4 in Acetonitrile
Gradient:
Flow Rate: 1.75 mL/min
Temperature: Ambient
Detection: UV @ 254 nm
Sample: 1. Cefaclor
Sample Pretreatment
Reconstitute 1 mg of neat Cefaclor standard with 1 mL of 1 M
HCl. Heat at 60 ºC for 6 hours. Neutralize with 1 mL of 1 M NaOH.
Refrigerate until ready to inject.
Column:Kinetex 5 μm F5
Dimensions: 150 x 4.6 mm
Part No.: 00F-4724-E0
Mobile Phase:
20 mM Potassium Phosphate pH 2.5/Acetonitrile (85:15)
Gradient:
Injection Volume: 1 μL
Flow Rate: 1 mL/min
Temperature: 22 °C
Detection: UV @ 254 nm
Analytes:
1. Cefadroxil
2. Cefaclor
3. Cefalexin
4. Cephradine
Column:
Kinetex 2.6 μm PS C18
Dimensions: 50 x 2.1 mm
Mobile Phase:
A: Water with 0.1 % Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
Gradient:
Flow Rate: 0.5 mL/min
Temperature: Ambient
Detector: MS/MS SCIEX™ API 4000™
Sample:
22 Antibiotics (Retention on Kinetex PS C18)
1. Sulfaguanidine (0.48 min)
2. Lincomycin (1.86 min)
3. Sulfapyridine (2.16 min)
4. Sulfathiazole (2.19 min)
5. Marbofloxacin (2.19 min)
6. Sulfamerazine (2.25 min)
7. Ciprofloxacin (2.28 min)
8. Florfenicol (2.34 min)
9. Danofloxacin (2.34 min)
10. Enrofloxacin (2.36 min)
11. Sulfamonomethoxine (2.42 min)
12. Difloxacin (2.47 min)
13. Valnemulin (2.49)
14. Tylosin A (2.74 min)
15. Sulfamethoxazole (2.9 min)
16. Tiamulin (3.04 min)
17. Sulfaquinoxaline (3.16 min)
18. Trimethoprim (3.33 min)
19. Oxolinic Acid (3.42 min)
20. Flumequine (3.42 min)
21. Sulfadimethoxine (4.0 min)
22. Rifaximin (4.03 min)
Column:
Kinetex® 2.6 μm PS C18
Dimensions: 50 x 4.6 mm
Mobile Phase:
A: Water with 20 mM Potassium
Dihydrogen Phosphate (pH 4.6)
B: Acetonitrile
Gradient:
Flow Rate: 1.5 mL/min
Temperature: 23 °C
Detector: UV @ 235 nm
Sample: 1. Berberine
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